Table 1.
Primers used for this study
| Forward sequence | Reverse sequence | Tm | Size | |
|---|---|---|---|---|
|
Nle1 sequencing primers | ||||
|
Nle1E1/2 |
CTTGACTCCTCCGAACACGAG |
AAACACAGCCTGTCTGTAGGTGAG |
62 |
500 |
|
Nle1E3 |
GATTAAATTTGTCGCATGGTGGTA |
GTCTGTTACTTGCAACGTGAGTCC |
62 |
475 |
|
Nle1E4 |
TATTTCTCCTCAGGGAATGGAGAG |
CCACACTCAGTCCAGTATCTGCTT |
62 |
377 |
|
Nle1E5/6 |
CTGTGTTCTCCCTCACCTCTCC |
ATAGTAGGCCAAGCCGTTGCT |
62 |
557 |
|
Nle1E7 |
ACAGCCTTGCTCTGCTGTTAGAA |
GGACCAGCTGGACTCTTGGTATAA |
62 |
440 |
|
Nle1E8/9 |
TTCCTGATTCTTGCCTTATGTCAC |
AACCCTAACTAAGACAACCAAGAACAA |
62 |
544 |
|
Nle1E10 |
TGGAGTTGCATGTAAGCTTGTGT |
GTCACTAGCCCTAAAGATGCCATT |
62 |
488 |
|
Nle1E11/12 |
CCGGCCCAGGTACCTAGCTT |
ACCTACAGGTTCTCCCAGAGTCTCC |
62 |
498 |
|
Nle1E13 |
ACTTGATACTTGGCAGTAGGCACA |
CTCCTGCTATCCAGTGCAAGG |
62 |
570 |
|
Nle1 genotyping primers | ||||
|
Nle1 GSP |
GCTGTAATGTCCTGACTGT |
|
60 |
637 |
| cDNA 1 |
CTGTGTCGTACTCTTCAAGGTCAT |
CTGTGGAGTCATCTTCTCCATATC |
|
|
| cDNA 2 |
TCAGACGACTTCACCTTATTCCTG |
CAGTCAACAGCATATACCTCATCG |
62 |
351 |
|
Nle1 DNA |
TCTCCTTCAGCTCCTTCACTGT |
TCCAATGGTGGAGTATAGGGTATAA |
60 |
341 |
|
cDNA amplification primers | ||||
| 1st PCR |
ATATGGATCCGGCGCGCCGTCGACT24 |
ATATCTCGAGGGCGCGCCGGATCCT24 |
67 |
|
| 2nd PCR |
(NH2)ATATGGATCCGGCGCGCCGTCGACT24 |
(NH2)ATATCTCGAGGGCGCGCCGGATCCT24 |
67 |
|
|
Eed |
GTGTGACATTTGGTACATGAGGTT |
ACATTTATGATGGGTCAGTGTTGT |
60 |
148 |
|
TaqMan gene expression assay | ||||
| |
Assay ID |
Amplicon Size (bp) |
|
|
|
Trp53 |
Mm01731290_g1 |
119 |
|
|
|
Cdkn1a |
Mm04205640_g1 |
80 |
|
|
| Gapdh | Mm99999915_g1 | 107 | ||