FIG. 3.
TAF1 dependence of the SRC promoters. (A) tsBN462 and BHK-21 cells were transfected with a minimal SRC1A promoter reporter, 0.38 SRC1A-CAT (34), and grown at 33°C for 36 h. Following treatment with 1 μM TSA or 5 mM sodium butyrate, transfected cells were grown at 33°C (permissive) or 39°C (restrictive) for 18 h. CAT levels were subsequently determined. (B) tsBN462 cells were transfected with 0.38 SRC1A-CAT with or without CMV-hTAFII250. Following 36 h of growth at 33°C, cells were shifted to 39°C and grown for an additional 18 h. CAT levels were subsequently determined. (C) tsBN462 and BHK-21 cells were transfected with a minimal SRC1α promoter reporter, −145 SRC1α-CAT (5), and grown at 33°C for 36 h. Following treatment with 1 μM TSA or 5 mM sodium butyrate, transfected cells were grown at 33 or 39°C for 18 h. CAT levels were subsequently determined. (D) tsBN462 cells were transfected with −145 SRC1α-CAT as described for panel B. Act, activity.