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. 2004 Mar;24(6):2560–2572. doi: 10.1128/MCB.24.6.2560-2572.2004

FIG. 4.

FIG. 4.

snf1Δ mutants are hypersensitive to HU, MMS, and cadmium. Wild-type (WT) (MCY2649), snf1Δ (MCY2916) transformed when indicated with pCE101-SNF1 expressing the WT Snf1 (5), pSNF1-HA expressing HA-tagged Snf1 (41), psnf1-T210A expressing the Snf1-T210A mutant (41), or the pRS316 empty vector, snf4Δ (MCY2634), sip1Δ sip2Δ gal83Δ (MCY4040), and snf1-K84R (MCY2693) strains were diluted to an optical density at 600 nm (OD600) of 0.2, and serial 10-fold dilutions were spotted on YPD plates (A) with HU or phleomycin at the indicated concentrations or on synthetic complete glucose medium (SC) plates (B) with (three upper lanes) or without (three lower lanes) uracil and containing HU at the indicated concentrations. (C and D) Serial 10-fold dilutions of the wild-type (MCY2649), snf1Δ (MCY2916), dun1Δ (CDY163), and mec1Δ sml1Δ (CDY196) strains were spotted on YPD plates containing the indicated concentrations of cadmium, HU, and MMS.