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. 2004 Mar;24(6):2560–2572. doi: 10.1128/MCB.24.6.2560-2572.2004

FIG. 7.

FIG. 7.

Genetic analysis suggests that Mig3 is a target of the Snf1 kinase in the cellular response to HU. (A) Log-phase culture of wild-type (WT) (MCY2649) and snf1Δ (MCY2916) cells transformed with either the vector pFL44 or pFL44-MIG3 (CDp20) were diluted to an OD600 of 0.2, and serial 10-fold dilutions were spotted and grown for 5 days on synthetic complete plates without uracil and containing HU at the indicated concentrations. (B) Log-phase culture of WT (MCY2649), snf1Δ (MCY2916), mig3Δ (CDY79), and snf1Δ mig3Δ (CDY80) were diluted to an OD600 of 0.2, and serial 10-fold dilutions were spotted and grown for 2 days on YPD plates containing HU at the indicated concentrations.