TABLE 3.
Antibody responses against the TI-1, TI-2, and TD antigens
| Antigen and genotype (n)a | Mean antibody production (relative titer [102]) ± SEMb
|
||||||
|---|---|---|---|---|---|---|---|
| IgM | IgG | IgG1 | IgG2a | IgG2b | IgG3 | IgA | |
| TI-1c | |||||||
| +/+ (8) | 11 ± 1.6 | 0.66 ± 0.15 | |||||
| −/− (7) | 16 ± 2.6 | 0.89 ± 0.21 | |||||
| TI-2d | |||||||
| +/+ (9) | 11 ± 1.1 | 4.2 ± 1.1 | |||||
| −/− (10) | 16 ± 1.7e | 9.5 ± 2.0e | |||||
| TDf | |||||||
| +/+ (3) | 4.7 ± 0.32 | 93 ± 6.8 | 16 ± 5.5 | 74 ± 22 | 9.4 ± 2.3 | 8.9 ± 2.2 | |
| −/− (5) | 3.9 ± 0.80 | 120 ± 33 | 11 ± 3.1 | 46 ± 14 | 7.9 ± 1.7 | 10 ± 2.8 | |
a
n, number of mice.
b
Statistical analysis was carried out by using Student's t test.
c
Mice were injected with the TI-1 antigen TNP-LPS, and the levels of hapten-specific IgM and IgG antibodies were measured at day 14 by ELISA.
d
Mice were injected with the TI-2 antigen TNP-Ficoll, and the levels of hapten-specific IgM and IgG3 antibodies were measured at day 14 by ELISA.
e
Significantly different from the value for +/+ mice at a P value of <0.05.
f
Mice were immunized with the TD antigen TNP-KLH, and the TNP-specific immunoglobulin subclasses were measured 7 days after the second immunization.