TABLE 4.
Decreased F-actin contents in APS−/− B-1 cells
| Cell type | Anti-IgM | Mean F-actin content (%) ± SEMa
|
|
|---|---|---|---|
| +/+ | −/− | ||
| B-1 | − | 100 | 92 ± 4.5 |
| + | 100 | 88 ± 2.8b | |
| B-2 | − | 100 | 97 ± 1.9 |
| + | 100 | 96 ± 2.9 | |
a
Antibody stained splenocytes or peritoneal cells were stimulated with anti-IgM (+) or left unstimulated (−), fixed, permeabilized, stained with rhodamine-phalloidin, and analyzed by flow cytometry. The average F-actin contents for −/− mice measured by fluorescence intensity for rhodamine-phalloidin are represented as the values relative to those for +/+ mice from four independent experiments. Statistical analysis was carried out by using Student's t test.
b
Significantly different from the value for +/+ cells at a P of <0.01.