Table 2.
Fatty acid composition and FA enzyme indices in the three different groups of rats
| SAT-TG | ||||||
|---|---|---|---|---|---|---|
| |
OP |
|
OR |
|
HFD-paired |
|
| |
Mean |
SD |
Mean |
SD |
Mean |
SD |
|
SCD-16 |
0.15#, †† |
0.03 |
0.11 |
0.04 |
0.12 |
0.02 |
|
SCD-18 |
7.6## |
0.7 |
7.0 |
0.7 |
6.5 |
0.6 |
|
D6Da |
2.1 *10-3 |
0.6 *10-3 |
2.3 *10-3 |
0.9 *10-3 |
2.4 *10-3 |
1.3 *10-3 |
|
D5D |
6.5 |
1.2 |
6.3 |
1.3 |
5.7 |
1.3 |
|
DNL |
0.94 |
0.06 |
0.89 |
0.06 |
0.88 |
0.05 |
|
EI |
0.22## |
0.02 |
0.24 |
0.04 |
0.27 |
0.03 |
|
16:1n-7 |
3.43#, †† |
0.67 |
2.49 |
0.83 |
2.63 |
0.44 |
|
18:2n-6 |
24.76#, †† |
1.07 |
25.94 |
0.67 |
25.69 |
0.83 |
|
18:3n-3a |
1.47## |
0.07 |
1.47‡‡ |
0.12 |
1.35 |
0.05 |
|
20:5n-3 |
0.03 |
0.01 |
0.03 |
0.01 |
0.02 |
0.00 |
|
22:6n-3 |
0.10# |
0.03 |
0.09 |
0.02 |
0.07 |
0.02 |
|
PL-CE | ||||||
| |
OP |
|
OR |
|
HFD-paired |
|
| |
Mean |
SD |
Mean |
SD |
Mean |
SD |
|
SCD-16 |
0.11 |
0.02 |
0.09 |
0.02 |
0.10 |
0.03 |
|
SCD-18 |
7.7 |
1.2 |
7.9 |
1.5 |
7.8 |
1.8 |
|
D6D |
15 *10-3 |
2 *10-3 |
14 *10-3 |
3 *10-3 |
16 *10-3 |
4 *10-3 |
|
D5D |
271 |
23 |
277 |
27 |
248 |
55 |
|
DNLa |
0.43 |
0.06 |
0.40 |
0.03 |
0.40 |
0.05 |
|
EI |
0.13 |
0.02 |
0.13 |
0.03 |
0.16 |
0.03 |
|
16:1n-7 |
0.75 |
0.12 |
0.65 |
0.12 |
0.74 |
0.22 |
|
18:2n-6a |
16.77 |
1.43 |
17.80 |
1.67 |
18.04 |
2.76 |
|
18:3n-3 |
0.13# |
0.03 |
0.13‡ |
0.03 |
0.17 |
0.04 |
|
20:5n-3 |
0.16 |
0.02 |
0.13‡ |
0.03 |
0.18 |
0.04 |
|
22:6n-3 |
1.37 |
0.14 |
1.47 |
0.21 |
1.27 |
0.20 |
|
PL-PL | ||||||
| |
OP |
|
OR |
|
HFD-paired |
|
| |
Mean |
SD |
Mean |
SD |
Mean |
SD |
|
SCD-16 |
0.012 |
0.002 |
0.011 |
0.003 |
0.011 |
0.003 |
|
SCD-18 |
0.19 |
0.03 |
0.21 |
0.03 |
0.22 |
0.03 |
|
D6Db |
3.4 *10-3 |
0.8 *10-3 |
3.2 *10-3 |
0.4 *10-3 |
N/A |
N/A |
|
D5D |
43 |
6 |
45 |
5 |
39 |
9 |
|
DNL |
0.92 |
0.13 |
0.90 |
0.07 |
0.95 |
0.24 |
|
EIa |
1.85### |
0.19 |
1.74‡‡ |
0.24 |
1.53 |
0.061 |
|
16:1n-7 |
0.18 |
0.03 |
0.18 |
0.04 |
0.20 |
0.05 |
|
18:2n-6 |
17.76 |
2.95 |
18.50 |
1.80 |
20.06 |
3.33 |
|
18:3n-3a |
0.08 |
0.02 |
0.06 |
0.02 |
0.09 |
0.03 |
|
20:5n-3a |
0.08 |
0.01 |
0.09 |
0.01 |
0.10 |
0.02 |
|
22:6n-3 |
4.22# |
0.46 |
4.18‡ |
0.74 |
3.48 |
0.62 |
|
Liver PL | ||||||
| |
OP |
|
OR |
|
HFD-paired |
|
| |
Mean |
SD |
Mean |
SD |
Mean |
SD |
|
SCD-16 |
0.013 |
0.004 |
0.011 |
0.003 |
0.011 |
0.004 |
|
SCD-18a |
0.16 |
0.02 |
0.16 |
0.03 |
0.16 |
0.03 |
|
D6Da |
12 *10-3 |
1 *10-3 |
11 *10-3 |
2 *10-3 |
12 *10-3 |
2 *10-3 |
|
D5Da |
58 |
7 |
61 |
6 |
58 |
17 |
|
DNL |
2.0 |
0.1 |
2.0 |
0.2 |
2.0 |
0.4 |
|
EI |
1.9 |
0.1 |
1.9 |
0.2 |
1.7 |
0.1 |
|
16:1n-7 |
0.18 |
0.03 |
0.18 |
0.04 |
0.20 |
0.05 |
|
18:2n-6 |
7.90 |
0.78 |
7.85 |
1.02 |
8.93 |
1.61 |
|
20:5n-3 |
0.07 |
0.01 |
0.07 |
0.01 |
0.08 |
0.02 |
|
22:6n-3 |
7.84 |
0.96 |
8.51‡‡ |
1.23 |
7.08 |
0.79 |
|
Liver TG | ||||||
| |
OP |
OR |
HFD-paired |
|||
| |
Mean |
SD |
Mean |
SD |
Mean |
SD |
|
SCD-16a |
0.047 |
0.020 |
0.040 |
0.008 |
0.039 |
0.013 |
|
SCD-18 |
14 |
1 |
13 |
1 |
13 |
1 |
|
D6D |
15 *10-3 |
2 *10-3 |
15 *10-3 |
2 *10-3 |
15 *10-3 |
2 *10-3 |
|
D5D |
6.8 |
0.5 |
6.9 |
0.7 |
6.8 |
1.0 |
|
DNLa |
1.1 |
0.3 |
1.0 |
0.1 |
1.0 |
0.1 |
|
EIa |
0.10 |
0.00 |
0.11 |
0.01 |
0.10 |
0.01 |
|
16:1n-7a |
1.28 |
0.66 |
1.01 |
0.22 |
1.03 |
0.36 |
|
18:2n-6a |
25.12 |
2.99 |
25.90 |
1.67 |
26.39 |
2.05 |
|
18:3n-3 |
0.85 |
0.15 |
0.83 |
0.11 |
0.85 |
0.12 |
|
20:5n-3 |
0.18 |
0.06 |
0.17 |
0.04 |
0.17 |
0.03 |
| 22:6n-3a | 1.15 | 0.43 | 1.16 | 0.42 | 1.02 | 0.35 |
Fatty acid (FA) composition and FA enzyme indices in five lipid fractions in obesity-prone (OP) and obesity-resistant (OR) rats with ad libitum HFD access (AL-HFD), and energy-restricted HFD-paired rats (HFD-paired). FAs are presented as relative percentages of total FAs within each lipid fraction. The symbol # indicates a significant difference between OP and HFD-paired; † between OP and OR; ‡ between OR and HFD-paired. Level of significance: #/†/‡, P < 0.05; ##/††/‡‡, P < 0.01; ###/†††/‡‡‡, P < 0.001. N/A, not available. All tests were done using One-Way ANOVAs, followed by Tukey’s t-test when significant; or Kruskal Wallis test (for non-normally distributed data; indicated by a superscript “;a” where performed), followed by Dunn’s multiple comparison test when significant. Where data for only two groups was available, Student’s t-test was used (indicated by a superscript “;b” where performed) for normally distributed data.