FIG. 9.

Tec, but not Itk or Btk, is localized in clusters at the immunological synapse of the D10 T-cell clone. The D10 T-cell clone was transfected with GFP-tagged Tec, Itk, or Btk. Twenty hours posttransfection, conjugates were formed between D10 cells and CH27 APCs plus cognate peptide, and the cells were fixed on glass slides and permeabilized. To confirm the formation of productive conjugates, cells were stained with PKC-θ rabbit antiserum, followed by Cy5-conjugated anti-rabbit antibody, and imaged by florescence microscopy with deconvolution software. Representative conjugates are presented as mid-plane images with GFP in green and PKC-θ in red.