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. 2013 Jan 29;8(1):e55064. doi: 10.1371/journal.pone.0055064

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© 2013 Klein et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) c-peptide⁺/7AAD⁻ and glucagon⁺/7AAD⁻ dissociated human islet cells were isolated by FACS. The right panel shows the flow cytometric analysis of the isolated populations. (B) Immunostaining of islet cells before and after FACS. C-peptide positive cells are shown in green and glucagon expressing cells in red.