Figure 2. Fixation and permeabilization of human islets does not affect the content of miRNAs.
Human islets were fixed and permeabilized as they were for FACS analysis. miRNAs expression was determined by qRT-PCR and fold changes (treated vs untreated islets) were expressed as mean +/− SD (n = 4). Control bar representing untreated islets is equal 1 also marked by the red dotted line. Paired, two-tail t test revealed no statistically significant differences in miRNAs expression (p>0.05).