Figure 6. Effect of chitooligosaccharides on chitoporin ion currents.

A single channel of VhChiP was inserted in the artificial membrane in A. a fully open state. Then, chitooligosaccharide: chitobiose, -triose, -tetraose, -pentaose, and -hexaose were added on the cis side of the chamber to a final concentration of 80 µM. Control recordings were made with maltopentaose and maltohexaose at a concentration of 400 µM. Ion current fluctuations were monitored for 120 s at applied voltages of ±100 mV. Here, only ion traces for +100 mV are presented.