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. 2013 Jan 29;11(1):e1001474. doi: 10.1371/journal.pbio.1001474

Figure 4. Effect of GR24 on PIN1 protein dynamics in inflorescence stems.

Figure 4

(A) Real-time monitoring of GR24-induced PIN1 depletion from the basal PM in wild-type, max1, or max2. (B) Effect of the protein synthesis inhibitor cycloheximide (CHX) on GR24-induced PIN1 instability in wild-type. The GFP signal in longitudinal sections from inflorescence stems of 6-wk-old soil-grown plants harbouring PIN1:PIN1–GFP in either the wild-type, max1, or max2 genetic background was monitored. Sections were mounted with the vehicle control or 5 µM GR24. For (B), sections were pretreated with 50 µM CHX for 30 min before addition of the vehicle control or 5 µM GR24 for 60 min. Means ± s.e.m. of the basal PM region of 7–9 cells are shown as a percentage of the value just after mounting. The vehicle control versus GR24-treated samples were compared by one-tailed Student's test. Results presented are typical of three independent experiments.