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. 2013 Jan 29;8(1):e51700. doi: 10.1371/journal.pone.0051700

Figure 4. Expression of Bmp4 in Dlx5;Dlx6 DKO limbs.

Figure 4

A-D. Detection of Bmp4 mRNA by WMISH on WT (left) and Dlx5;Dlx6 DKO mutant (right) limbs, at E11. FLs are on the top, HLs are on the bottom. E,F. In situ detection of Bmp4 mRNA in the pharyngeal arches region of WT (left) and Dlx5;Dlx6 DKO mutant (right), at E11, as a control for RNA preservation. G-L. Detection of Gremlin mRNA in FLs (G,H) and HLs (I-L) of WT (left) and Dlx5;Dlx6 DKO mutant embryos (right), at E10.5. While Bmp4 expression in the anterior mesoderm of the FLs (A,B) or the HLs (C,D) is unchanged (green arrowheads), expression in the central wedge of the AER of mutant embryos is diminished in the HLs, but not in the FLs (red arrows in D). Gremlin expression is unchanged both in the FLs (G,H) and in the HLs (I-L) of Dlx mutant embryos (red arrowheads). Genotypes and probes are reported on the top. The Anterior-Posterior (A-P) orientation is indicated. M,N. whole-mount photographs documenting the reduced size of mutant embryos and justifying the slightly reduced size of the mutant limbs, often observed. O. Quantification of Bmp2 and Bmp4 mRNAs by qRT-PCR in the anterior and posterior halves of HLs from embryos with the genotype indicated on the top of each graph, compared to WT.