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. 2013 Jan 29;8(1):e51700. doi: 10.1371/journal.pone.0051700

Figure 5. Binding of DLX5 on predicted conserved elements close to BMP2 and BMP4. A.

Figure 5

Location of predicted conserved Dlx binding sites in regions of the mammalian genome around the BMP2 (top) and BMP4 (bottom) loci. Sites are indicated with colour vertical bars, the chromosomal position and coordinates are shown. The mammalian genomic conservation is reported on the bottom. With the exception of B2-RE3, all elements fall within stretches of conserved sequences. B. Sequences and alignment of the predicted Dlx binding elements. The sequence corresponding to the PWM is shown in red. Dashed lines indicate the degenerated part of the binding sequence. C. Western blot analysis to demonstrate expression of DLX5-myc and DLX5-Q184P-myc proteins in U2Os cells. The molecular weight of the detected proteins is indicated on the left. D. ChIP analyses on the predicted Dlx binding sites in the human U2Os cells, transfected with the DLX5-myc and DLX5-Q178P-myc expression vector, or with the control empty vector. The input chromatin (positive control) is shown on the left, ChIP with or without anti-myc are shown in the mid panels. ChIP with and without anti-H4Ac on the same elements are shown on the right.