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. 2013 Jan 29;8(1):e54605. doi: 10.1371/journal.pone.0054605

Figure 6. Transcript and protein expression levels of β-glucoronidase/Gusb and neprilysin/Mme in the hippocampus and cerebellum of five lines of mice.

Figure 6

A and C, Histograms showing increased mRNA levels for Gusb (A) in both hippocampus and cerebellum and Mme (encoding neprilysin, C) in the cerebellum of Npc1-null and ANPC mice compared with WT control mice as obtained using customized real-time RT-PCR array. B and D, Immunoblotting performed to validate data obtained by PCR arrays revealed increased levels of Gusb (B) in both the hippocampus and cerebellum and neprilysin (D) in the cerebellum of Npc1-null and ANPC mice compared with WT mice. APP-Tg and Dhet mice showed no significant alteration in transcript or protein expression levels of Gusb (A, B) or neprilysin (C, D) compared with WT mice. The protein levels of Gusb and neprilysin were normalized to the β-actin and the values (n = 4 animals per genotype) are expressed as means ± SEM. *, p<0.05.