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. 2013 Jan 29;8(1):e54605. doi: 10.1371/journal.pone.0054605

Figure 7. Transcript and protein expression levels of tau/Mapt and Abca1 in the hippocampus and cerebellum of five lines of mice.

Figure 7

A, Histograms showing decreased Mapt mRNA (encoding tau) level in the cerebellum but not in hippocampus of Npc1-null and ANPC mice compared with WT mice as obtained using customized real-time RT-PCR array. B, Immunoblots and respective histograms validating the decreased levels of tau in the cerebellum of Npc1-null and ANPC mice compared with age-matched WT mice. C, Histograms showing increased Abca1 mRNA level in the hippocampus of Npc1-null and ANPC mice and in the cerebellum of Npc1-null mice compared with WT as obtained using customized real-time RT-PCR array. D, Immunoblotting performed to validate data obtained by PCR arrays revealed significant up-regulations in the Abca1 protein level in Npc1-null and ANPC mice in the respective brain regions compared with WT mice. The protein levels of tau and Abca1 were normalized to those of β-actin and Gapdh respectively, and the values (n = 4 animals per genotype) are expressed as means ± SEM. *, p<0.05.