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. 2013 Jan 29;8(1):e54750. doi: 10.1371/journal.pone.0054750

Figure 5. Chronic ethanol ingestion modifies Cys thiols on α-ENaC.

Figure 5

A) Left panel F5M labeling of α-ENaC protein immuno-precipitated from chronic ethanol fed mice (left lane) or maltodextrin control animals (right lane). Middle panel shows bioluminescent detection of α-ENaC using the same membrane shown in left panel. Right panel Co-registration of F5M and ENaC signal (ENaC signal is pseudo-colored and F5M signal contrast reduced in order to enhance co-registration of data obtained from the same blot). B) Normalization of F5M RLU to ENaC expression levels; n = 3; p<0.05.