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. 2013 Jan 29;8(1):e54750. doi: 10.1371/journal.pone.0054750

Figure 7. Chronic ethanol ingestion alters protein expression in the lung.

Figure 7

A) Normalized α-ENaC mRNA levels are elevated in C57Bl/6 mouse lung chronically ingesting 20% v/v EtOH. Data represents 9 independent observations from 3 animals in EtOH and isocaloric control groups. B) Expression of α-ENaC protein is significantly increased in mice chronically ingesting 20% w/v EtOH. Ave of data reported from n = 3 mice from EtOH and isocaloric control groups. Data normalized to β-actin expression levels, and p<0.0001. C) Representative western blot of C57Bl/6 mouse lung following chronic alcohol, or isocaloric control diets w/without 1 mg/mL LPS inoculation. D,E) Densimetric analysis show NOX2 catalytic domain gp91phox (n = 3) and Rac1 (n = 6) significantly increases following (chronic) EtOH and (acute) LPS treatment. p<0.05 where indicated by asterisks.