Table 1.
Sequences of peptidesa
WALP19-P10 | acetyl-GWWLALALAPALALALWWA-ethanolamide |
GWALP23-P12 | acetyl-GGALWLALALAPALALALWLAGA-ethanolamide |
A16GWALP23-P12 | acetyl-GGALWLALALAPALAAALWLAGA-ethanolamide |
WALP19-P10 is included for reference. Labels were incorporated for 2H and 15N NMR experiments. Deuterium labels were introduced in two alanines per GWALP23-P12 peptide, using partial (p) and full (f) deuteration at the following positions: 7f and 9p, 13p and 15f, 17f and 21p; and 15p and 16f (in the mutant A16GWALP23-P12 sequence). Two GWALP23-P12 peptides were synthesized with uniform 15N enrichment, covering residues 13–17, or residues 6–11 together with 13–18.
[NB: please use a fixed-width font for the sequences in Table 1, and align them with respect to the central “P” as shown.]