FIG. 6.

Nup358/RanBP2 knocked-down cells are less efficient in CRM1-mediated export of Rev-GFP. (A) Rev-GFP-transfected MCF-7 cells were scored for predominant accumulation of GFP in the cytoplasm (Nuc<Cyto), the nucleus (Nuc>Cyto), or equal distribution (Nuc=Cyto) between the two compartments under control (black bars) or Nup358 RNAi (white bars) conditions. The mean distribution in three independent experiments is shown; error bars represent standard errors. Illustrative images of Nup358 show a strong (C1 and C2) and weak (D1 and D2) export defect. Empty pSUPER-transfected cells are shown in panels B1 and B2.