FIG. 2.

The C-terminal region is responsible for Lub1 functions. (A) Schematic representation of Lub1 truncated mutants. The ability of each Lub1 deletion mutant to suppress the temperature sensitivity of Δlub1 cells is summarized. +, positive; −, negative; WD, WD repeat domain; *, mutation site. (B and C) Identification of the dosage-dependent suppressor of the Δlub1 phenotype. Δlub1 cells harboring the expression vector for the indicated proteins were streaked onto EMM plates and incubated at 36°C (B) or UV irradiated at the doses indicated and incubated at 27°C (C) as described for Fig. 1. Ub, FLAG-ubiquitin; K48R and K63R, FLAG-ubiquitin (K48R and K63R) mutants; WT, wild type.