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. Author manuscript; available in PMC: 2014 Feb 12.
Published in final edited form as: Neuroscience. 2012 Nov 29;231:28–37. doi: 10.1016/j.neuroscience.2012.11.034

Figure 2. Sox11 and TANK are upregulated in DRG following nerve injury.

Figure 2

A. Sox11 mRNA is increased in lumbar DRG in response to sciatic nerve axotomy (n = 4 per group, p < 0.001 vs. naïve, ANOVA with Bonferroni post-hoc test). B. RT-PCR products run on an ethidium bromide labeled agarose gel show increase in TANK mRNA variants. The larger TANK mRNA variant 3 (at 685 bp) is preferentially increased in lumbar DRG at 1D and 4D post axotomy. T, total TANK mRNA; V3, variant 3 mRNA. C. Western blot shows upregulation of TANK band at ~53kDa (indicted by asterisk) in DRG at 1d and 3d post axotomy, relative to naïve control (C). Arrowhead indicates 48 kDa isoform. D. Densitometric analysis using larger groups (n=8 mice per group) show the 48kDa isoform also increases in response to injury at 3d post-injury (38%, p < 0.05, vs. naïve, Student’s t-test) in DRG in response to axotomy. Values were normalized to GAPDH expression. E. siRNA-mediated knockdown of Sox11 in cultured DRG neurons reduces intensity of the TANK immunoreactive band at ~53kDa (arrowhead) relative to cultures treated in parallel with nontargeting (NT) siRNA. GAPDH is shown as loading standard. Asterisks on charts indicate significance.