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. Author manuscript; available in PMC: 2014 Feb 12.
Published in final edited form as: Neuroscience. 2012 Dec 5;231:282–295. doi: 10.1016/j.neuroscience.2012.11.049

Fig. 3.

Fig. 3

Western blot analysis with ProteinTech affinity-purified rabbit anti-THAP1 polyclonal antibody. (A) Signal specificity was evaluated in HEK293 protein extracts without and with antibody pre-absorption. THAP1 was also detected with mouse anti-V5 and Novus affinity-purified polyclonal rabbit anti-THAP1 antibodies. β-tubulin served as the endogenous control. (B) A single band was seen with protein extracts from striatum, thalamus, hippocampus, cerebellum and cerebral cortex. β-tubulin was the endogenous control. (C) Immunohistochemical staining patterns in P14 and 2M rat cerebellum were similar with ProteinTech and Novus anti-THAP1 antibodies. Scale bar, 100 μm. (D) Relative THAP1 expression in comparison to 2M cerebral cortex with β-tubulin as the endogenous control.