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. Author manuscript; available in PMC: 2014 Jan 25.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Dec 19;430(4):1277–1282. doi: 10.1016/j.bbrc.2012.11.137

Fig. 2. MG132 induces NAG-1 expression in a concentration and time-dependent manner.

Fig. 2

(A–C) U87MG cells were treated with the indicated concentrations of MG132 for 12 h. (D–F) U87MG cells were treated with 1 μM MG132 for the indicated amount of time. (A and D) Quantitative RT-PCR shows the NAG-1 mRNA levels. * p < 0.05. (B and E) Western blotting analysis depicts the 35-kDa pro-form of the NAG-1 protein and β-actin expression. (C and F) ELISA analysis was used to measure NAG-1 secreted into the media. Values are mean ± SD of three independent experiments. * p < 0.05.