FIG. 2.
eIF2BβV341D exhibits a reduced growth rate and a defect in global translation. (A) The doubling times of strains containing the indicated alleles were determined in YPD liquid medium during exponential growth. CACH mutant strains were derived from KAY16 (eIF2Bɛ alleles) or H2217 (eIF2Bβ alleles). a, for purposes of comparison, the doubling time for the eIF2Bβ-201 strain (H1725) and its isogenic parent (H1727) are shown. High copy indicates 2μm plasmid-borne alleles. WT, wild type; ND, not determined; NA, not applicable (B) Growth curves of wild-type and isogenic eIF2BβV341D mutant strains used to generate the doubling time shown in panel A. (C) Polysome profile analyses of wild-type eIF2B, eIF2BβV341D (in strain H2217), and eIF2Bβ-201 (strain H1793) mutants cultured in YPD medium at 30°C. 40S, 60S, 80S, and polyribosome peak positions are indicated. The polysome/monosome ratio (P:M) was quantified for each trace by using NIH Image v1.61 software.