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Overexpression of eIF2Bδ rescues the growth defect of eIF2BβV341D cells. (A) Growth of liquid SC-uracil cultures. The calculated doubling time (Td) in liquid exponential-phase culture is indicated. Strains are gcn2Δ eIF2BβV341D cells transformed with wild-type eIF2Bβ (•), high-copy-number (HC) eIF2Bδ (▴), or empty vector control (▪). (B) Serial dilutions of the same strains on solid SD ± 3AT. (C) Growth of the same strains on an AZC gradient.
