FIG.1.

Sequence analysis of Dof and related proteins. (A) Aligment over the DBB motif and the ankyrin repeats of Drosophila Dof (accession number O96757), Anopheles Dof (accession number Q8T5J9), mouse BCAP (accession number Q9EQ32), chicken BCAP (accession number AAG48583), human BCAP (accession number XP_058343), fugu BCAP (ENSEMBL SINFRUP00000078060), lamprey BCAP (accession number AAN64296), human BANK (accession number Q8WYN5), and Ciona intestinalis BCAP (a translation of accession number AK115423). The alignment begins at positions 216 of Drosophila Dof, 395 of Anopheles Dof, 182 of mouse BCAP, 3 of human BCAP, 180 of the predicted fugu BCAP sequence, 178 of lamprey BCAP, 170 of human BANK, and 1 of the incomplete Ciona BCAP sequence. Six or more identical or similar residues shared by the sequences are shown in red and blue, respectively, and are marked below the alignment by a dot. An asterisk denotes a position that is absolutely conserved. (B) Alignment of A. gambiae Dof and Drosophila Dof was made using CLUSTAL W, and regions representing insertions in the Anopheles sequence were removed. Then, the number of identities found within a window of 15 amino acids was plotted for each position of the Drosophila protein. The locations of the DBB motif, the ankyrin repeats, and the coiled coil within Drosophila Dof are indicated at the top of the chart. The positions of insertions and deletions within the Anopheles sequence are shown below the chart as arrows pointing toward and away from the sequence of the Drosophila protein, respectively. The numbers represent the lengths of the insertions and deletions.