FIG. 4.

Subcellular localization of mutant Dof proteins and influence of Dof on localization of ectopically expressed Htl. (A to L) The stabilities and subcellular localization of mutant forms of Dof were assessed using a prd-Gal4 transgene to express each construct in ectodermal cells. The expression and subcellular localization of the mutant proteins were followed by staining fixed embryos with an anti-Dof antiserum. (M to O) A transgene encoding Htl was expressed using prd-Gal4 either alone (M) or in combination with a transgene encoding Dof with an N-terminal flag epitope tag (N and O). The subcellular distribution of Htl (M and N) was visualized by staining the embryos with an anti-Htl antiserum, and the localization of Dof (O) was determined as described above. All embryos depicted are of the same age (stage 10). The orientations of the embryos are shown in the top right corner of each panel; the white boxes indicate the regions (39 by 35 μm) that were photographed at high magnification. The proteins ectopically expressed are indicated in each panel.