Table 1.

Primer sequences used in polymerase chain-based assays, product size and annealing temperature

	Primer sequence	Size (bp)	Temp (°C)
First PCR	(F)5’-GTTAAGGGGTTTAGGGTTGGA	183	60
Nested PCR	(R)5’-CAAAAACTTATATTAAAAAATAAAC
Methylated	(F) 5’-GGAGTTGTTTCGATAGGAGCGC	71	60
	(R) 5’-TTACTAAAACCGAACTAAACGCG
Unmethylated	(F) 5’-GGAGTTGTTTTGATAGGAGTGT	71	60
	(R) 5’-TTACTAAAACCAAACTAAACACA

Temp: Temperature; PCR: Polymerase chain reaction; R: Reverse; F: Forward.