FIGURE 2.

Mapping the spatial distribution of complexes of Wzm and Wzt proteins in thin (1.5 µm) optical sections through a CHO cell co-expressing Wzm proteins tagged with GFP₂ as a donor, and Wzt proteins tagged with YFP as an acceptor. Two-dimensional spatial distribution maps of fluorescence of donors in presence of acceptors (k^DA) and the fluorescence of acceptors in presence of donors (k^AD) were obtained from spectral unmixing, while the apparent FRET efficiency (E_app) map was calculated from k^DA and k^AD for every pixel (see text for details).