FIGURE 4.
Homo-oligomerization of Wzt expressed in CHO cells in the presence of untagged Wzm. A. Apparent FRET efficiency (Eapp) maps of representative cells expressing Wzt in the presence of Wzm. B. Distributions of FRET efficiencies (Eapp histograms) across the pixels composing the FRET efficiency maps shown in panel A. C. Meta-histogram obtained by accumulating the peak positions of 87 cells presenting single or dominant peaks in their Eapp distributions (circles) and theoretical best-fit to three correlated Gaussians shown individually (red, green, and brown lines) or as a sum (black line). The three peaks in the meta-histogram correspond to different configurations of a square-shaped tetramer shown in the same order in panel D. The best-fit was obtained for Ep ± S.D. equal to 0.17 ± 0.016. D. Square-shaped tetramer model used to interpret the results in panel C. Ep is the pair-wise FRET efficiency between a single donor (D) and a single acceptor (A).