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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: FEBS J. 2013 Jan 2;280(3):827–839. doi: 10.1111/febs.12072

Figure 2. Adeno-associated virus (AAV) transduction of C2C12 myoblasts.

Figure 2

(A) Cells were plated at low densities, 500, 1000 and 2000 cells/well (~130, 260 & 520 cells/cm2, respectively), and incubated for 5 days with AAV2/cmvGFP (108 vg/well). Transduction efficiency (mean % +/− SEM; no significant differences) is indicated for each treatment and was determined by counting fluorescent and total cell numbers in 9 random views from each well/treatment. (B) The 1000 cell group was then diluted 5-fold, passed into new wells and subsequently cultured for 96 h in virus-free medium. After 72 h, some cells were treated with 1 µM TSA for an additional 12 h before imaging at 100×. Transduction efficiency (mean % +/− SEM; different letters = significant difference, same letters = no difference; A & B analyzed separately; p≤0.05) is indicated for each treatment and was determined by counting fluorescent and total cell numbers in 9 random views from each well/treatment.