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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: FEBS J. 2013 Jan 2;280(3):840–854. doi: 10.1111/febs.12073

FIGURE 3.

FIGURE 3

DAF 3’-UTR contains destabilizing elements between nucleotides 1860 and 2100. (A) DAF 3’-UTR was examined for AU rich elements (ARE) using the programme AREsite (http://rna.tbi.univie.ac.at/AREsite). The AU rich regions are indicated by italicized letters. The putative binding sites for elav family protein HuR are indicated by underscores and bold letters. Letters with only underscores indicate WWAUUUAWW type of HuR binding sites. Letters with underscores and bold indicate WUAUUUAUW type of HuR binding sites. (B) Systematic representation and cloning strategy of DAF gene 3’-UTR into pβgalc reporter vector. (C) DAF 3’-UTR deletions were cloned into pβgalc reporter vector and transfected to Ishikawa cells. Cells were incubated for 48 h, and β- galactosidase activity was measured. Experiment was performed three times, and one set of data is presented. Error bars indicate S.E of triplicate samples (*, **, p < 0.001 versus UTR 1.35).