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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: FEBS J. 2013 Jan 2;280(3):840–854. doi: 10.1111/febs.12073

FIGURE 6.

FIGURE 6

Invitro binding of rHuR to DAF mRNA ARE is altered by NO. (A) Nucleotide sequence of 5’-tagged fragment of DAF mRNA in the 3’-UTR-AU rich region containing putative HuR binding sites. (B) Recombinant HuR (8.7 nM) was exposed to DTNO (1 mM) for 1 h and excess and spent DTNO was removed by passing through desalting column. Equal volume (20 µl) of control and DTNO exposed rHuR was added to DAF mRNA fragment attached to streptavidin coated wells in a microplate. rHuR bound to DAF mRNA was measured using anti-HuR antibody and secondary antibody conjugated to HRP. Experiment was performed three times and one set of data is presented.