TABLE 1.

Relative dissociation constants for wild-type and mutant forms of TFIIIA^a

Mutation	Avg relative dissociation constant ± SE
	Somatic-type gene	Oocyte-type gene	5S rRNA
S16A	0.41 ± 0.04	0.48 ± 0.03	0.91 ± 0.04
S16E	1.08 ± 0.04	1.09 ± 0.14	0.97 ± 0.08
S314A	1.03 ± 0.22	0.95 ± 0.22	1.17 ± 0.34
S314E	4.56 ± 0.38	3.76 ± 0.79	0.51 ± 0.08
S328A	NDb	ND	0.79 ± 0.28
S328E	ND	ND	0.85 ± 0.09

^aBinding is expressed as the ratio of the apparent dissociation constant of mutant TFIIIA relative to that of wild-type TFIIIA.

^bND, not determined