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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Microbiol. 2012 Oct 9;86(4):971–987. doi: 10.1111/mmi.12038

Figure 4.

Figure 4

In trans expression of HvJAMM1stimulates the desampylation of SAMP1 (A) and SAMP2 (B) conjugates and is stimulated by temperature upshift of stationary phase cells to 50 °C. H. volcanii HM1096 (Δsamp1 Δsamp2 Δhvo_2177) was used as parent with plasmids pJAM202c (empty vector), pJAM947 (Flag-SAMP1), pJAM1810 (Flag-SAMP1 and HvJAMM1), pJAM1811 (Flag-SAMP1 and HvJAMM1 E31D), pJAM949 (Flag-SAMP2), pJAM1812 (Flag-SAMP2 and HvJAMM1), pJAM1814 (Flag-SAMP2 and HvJAMM1 E31D). HvJAMM1 wild type (wt) and inactive variant (E31D) are indicated. Strains were grown in ATCC974 medium at 42 °C for 60 h and shifted to 50 °C or maintained at 42 °C for 12 h. Cell pellets were boiled in reducing SDS-PAGE lysis buffer. Proteins were separated by SDS-PAGE and analyzed by α-Flag immunoblot.