FIG. 2.

Differential binding of the SHIP-1 or Csk SH2 domain to tyrosine-phosphorylated Dok-3. Cos-1 cells were transfected with the indicated dok-3 cDNAs in the presence or absence of FynT Y528F. Equivalent amounts of total cell proteins were then incubated with either GST-SHIP-1 SH2 domains (A) or GST-Csk SH2 domains (B). After several washes, the association with Dok-3 was detected by immunoblotting with anti-Dok-3 (top blocks). The expressions of Dok-3 (middle blocks) and FynT (bottom blocks) were verified by immunoblotting of total cell lysates with anti-Dok-3 and anti-FynT, respectively.