FIG. 2.

Activation of Jak2 and Stat5 in transfected cell lines in response to Epo stimulation. DA3 and CTLL clones expressing the murine EpoR-human γ-like chimeric receptor (mEpoR/hγ-like) were used. Transfected cells were starved overnight and treated with the indicated stimuli or not treated (−). Lysates were prepared and subjected to immunoprecipitation (IP) with the anti-Jak2 antibody (αJak 2) and antibody against Stat5a and Stat5b (αSTAT 5A/B) shown. The activation of Jak2 (A) and Stat5 (B) was assessed by probing with antibodies to phosphotyrosine (αPTyr). Blots were stripped and reprobed with the indicated antibodies to assess protein levels.