Fig. 1.

Expression screening of various FMDV P1-2A ± 3C cassettes. (A) Cartoon representation of the genetic designs tested. The text near the bottom right represents the amino acid sequence of FMDV 3C between residues 141–170 with Cys 142 and Cys 163 highlighted. (B) Outcome of screening the various A22 Iraq constructs by immunoblotting using a polyvalent A serum. The expected migration positions of P1-2A-3C, P1-2A and VP1 are indicated although very little P1-2A-3C is visible. Numbers to the left are the migration positions of protein markers and are in kilodaltons.