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Construction of B. burgdorferi uvrABbu inactivation mutant and complementation plasmids. A. Generation of ΔuvrABbu inactivation mutant by substitution of part of uvrABbu with the kanamycin resistance gene aph(3′)-IIIa (KmR) from Enterococcus faecalis under its own promoter (Shevchuk et al., 2004). See Materials and Methods for details. B. Construction of plasmids for complementation of ΔuvrABbu inactivation mutant.
