TABLE 2.
Relevant genotype | Frequency of stable Ura+ (10−2) with CFV/D8B-tg | Frequency relative to wild type |
---|---|---|
RAD | 20 ± 10 | 100 |
rad52 | <0.0027 | <0.014 |
rad51 | 0.14 ± 0.05 | 0.70 |
rad54 | 0.20 ± 0.1 | 1.0 |
rad55 | 0.47 ± 0.3 | 2.4 |
rad57 | 0.52 ± 0.2 | 2.6 |
rad59 | 12 ± 7.7 | 60 |
rad51 rad59 | 0.07 ± 0.03 | 0.35 |
rad50 | 11 ± 0.4 | 55 |
rad50 rad51 | 0.47 ± 0.29 | 2.4 |
rad50 rad59 | 3.5 ± 1.6 | 18 |
rad50 rad51 rad59 | 0.34 ± 0.16 | 1.7 |
RAD/RAD | 17 ± 5.3 | 100 |
rad51/rad51 | 0.17 ± 0.04 | 1 |
The frequency of BIR is the number of stable Ura+ transformants per microgram with cut DNA divided by the number of transformants per microgram with uncut DNA transformed.