TABLE 3.
Effect of the rad50, rad51, and rad59 mutations on the frequency of BIRa at other loci
| Relevant genotype | Frequency of BIR at:
|
|||
|---|---|---|---|---|
| CFV/D8B-tg | CFV/MRC1-tg | CFV/YBR235-tg | CFV/PCA1-tg | |
| RAD | 20 ± 10 | 39 ± 19 | 6.3 ± 2.4 | 12.8 ± 9.4 |
| rad51 | 0.14 ± 0.05 | 0.95 ± 0.1 | 0.19 ± 0.05 | 0.36 ± 0.2 |
| rad50 | 11 ± 0.4 | 76 ± 19 | 8.9 ± 6.1 | 31 ± 23 |
| rad59 | 12 ± 7.7 | 27 ± 15 | 5.0 ± 0.7 | 17 ± 5.3 |
a
The frequency of BIR for each vector is the number of stable Ura+ transformants per microgram with cut DNA divided by the number of transformants per microgram with uncut DNA transformed (10−2).