Fig. 7.

Normal paired-pulse facilitation in TRPC5 KO, TRPC1 KO, and TRPC1/4 DKO mice. (A) Representative traces of paired-pulse facilitation (PPF) of Schaffer collateral field excitatory postsynaptic potential (fEPSP) in WT, TRPC5 KO, TRPC1KO, and TRPC1/4 DKO mice. A pair of electric stimuli with increasing intervals (40, 80, 120, 160, 200, 240, 280, and 320 ms) was delivered at 10-second intervals, and the resulting pair of fEPSPs was recorded. (B–D) The averaged PPF ratios (the peak of the second EPSP over the peak of the first EPSP in each pair) and standard errors were plotted (n = 5-15, 5-13, 6, 6 for WT, TRPC5 KO, TRPC1 KO and TRPC1/4DKO, respectively). Note the peak of PPF occurs around 40–50 ms intervals and the subsequent exponential decay at greater intervals. There was no statistically significant difference between WT and TRPC5 KO mice, between WT and TRPC1 KO mice, or between WT and TRPC1/4DKO mice (two-way ANOVA).