TABLE 4.
Effect of allosteric modulators on saturation binding of [3H]CP55940 in mouse brain membranes and hCB1 cell membranes
Data shown are mean with 95% CI of three to six independent experiments.
| Kd | Bmax | |
|---|---|---|
| (95% CI) | (95% CI) | |
|
nM |
pmol/mg |
|
| Mouse Brain Membranes | ||
| [3H]CP55 + Vehicle | 2.67 (1.5–3.8) | 1.59 (1.3–1.8) |
| [3H]CP55 + Org (1 μM) | 2.16 (1.5–2.8) | 2.43 (2.2–2.7)† |
| [3H]CP55 + Org (10 μM) | 2.76 (1.7–3.8) | 3.25 (2.8–3.7)† |
| [3H]CP55 + PSN (1 μM) | 6.00 (3.1–8.9) | 3.64 (2.7–4.5)† |
| [3H]CP55 + PSN (10 μM) | 5.66 (3.6–7.7) | 2.98 (2.4–3.5)† |
| hCB1 | ||
| [3H]CP55 + Vehicle | 0.45 (0.26–0.63) | 6.4 (5.5–8.4) |
| [3H]CP55 + PSN (1 μM) | 0.56 (0.45–0.66) | 11 (10.8–12.2)† |
†
Indicates the values are significantly different from vehicle as indicated by non-overlapping confidence limits.