Fig. 2.

NADS or NADPS treatment decreased steady-state levels of DHFR. (A) Western blot analysis of total lysates from DG44-(DHFR-EGFP) cells was performed after treatment with 0, 1, and 10 μM NADS for 48 hours. DHFR-EGFP fusion protein was detected using an antibody against EGFP. (B) CCRF-CEM and CCRF-CEM/R cells were exposed to 0, 1, and 10 μM NADS and NADPS for 0, 24, and 48 hours. The DHFR levels were detected using an anti-DHFR antibody. Equal loading was determined with GAPDH or α-tubulin, respectively.