Fig. 10.

Western blot analysis of human blood plasma samples treated with GD. (Top) Western blot of human blood plasma samples treated with GD (0.18 μM) for 72 hours and probed with mAb-HSA-GD. (Bottom) Ponceau staining of the PVDF membrane used in the Western blot shows an equal amount of serum albumin. Lane 1, untreated human plasma (1 μl); lane 2, human plasma (1 μl) treated with 0.18 μM GD; lane 3, positive control protein HSA-GD-adducted protein standard (1 μg).