Fig. 5.

Characterization of antibodies by competitive ELISA with synthetic phosphonylated and nonphosphonylated peptides attached to ovalbumin (i.e., HSA-GB-OVAL, HSA-GD-OVAL, HSA-GF-OVAL, HSA-VX-OVAL, and nonphosphonylated HSA-NP-OVAL). HSA-NP is the nonphosphonylated peptide (NP). (A) mAb-HSA-GD. (B) mAb-HSA-VX. ▼, HSA-VX; □, HSA-GD; △, HSA-GF; ○, HSA-GB; ◇, HSA-NP; RU, relative luminescence units. Experiments were carried out with 1:100-diluted hydridoma cell culture medium. mAb was preincubated separately with each phosphonylated or nonphosphonylated HSA-peptide as described in Materials and Methods. The bound antibody was measured indirectly using an HRP-conjugated secondary antibody.