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. 2013 Feb;344(2):511–521. doi: 10.1124/jpet.112.199745

Fig. 1.

Fig. 1.

Controls for iNOS and eNOS in situ hybridization histochemistry. (A) Positive control image of a striatal section from a mouse with Theiler's murine virus (TMV) infection in the brain (indicated by the arrow) showing detection of iNOS mRNA induction using the antisense ribonucleotide probe described in this article. (B) Negative control image showing a lack of signal in a brain section from the same TMV-infected mouse hybridized with the sense ribonucleotide probe. (C) Positive control image of a rat brain section at the level of the dorsal hippocampus hybridized with the antisense ribonucleotide probe against eNOS mRNA used in the present study. (D) Control image of a rat brain section at the level of the dorsal hippocampus hybridized with the sense ribonucleotide probe against eNOS mRNA used in the present study. Scale bar = 2 mm, applies to all panels.