(A) Quantitative analysis of the effects of single or repeated METH exposure on nNOS activity in the striata of animals sacrificed 1 hour after the last injection on PND90. Treatment group designations indicate PND60 treatment:PND90 treatment, resulting in the four treatment groups: Saline:Saline (SS), METH:Saline (MS), Saline:METH (SM), and METH:METH (MM). Data are expressed as the percentage area of the field with signal above threshold. *Significant main effect of PND90 treatment, P < 0.05. (B) Representative image of NADPH diaphorase histochemical staining in an SM animal sacrificed 1 hour after the last injection on PND90. Scale bar = 50μm.