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. Author manuscript; available in PMC: 2013 Jan 30.
Published in final edited form as: Cancer Res. 2008 Jan 15;68(2):580–588. doi: 10.1158/0008-5472.CAN-07-3091

Figure 3.

Figure 3

CaMKKβ but not LKB1, is necessary for PIA-induced AMPK activation in cells. A, immortalized isogenic wild-type or LKB1−/− MEFs were treated with PIA5 for the indicated times, and phosphorylation of AMPK was assessed by immunoblotting. B, reconstitution of wt LKB1 into LKB1−/− MEFs. LKB1−/− MEFs were nucleofected with vector or wt LKB1 cDNA construct as described in Materials and Methods. Transfected cells were treated with vehicle or PIA5 for he indicated times. Expression of LKB1 and phosphorylation and expression of AMPK were assessed by immunoblotting. C, H1155 (left), H157 (top right), or A549 (bottom right) cells were pretreated with vehicle or STO-609, followed by treatment with vehicle or PIA5 for the indicated times. D, the CaMKKβ mutant lung cancer cell line H1770 was treated with PIA5 for the indicated times. Immunoblotting in C and D was performed using antibodies that recognize the phosphorylated forms of AMPK, ACC, and p38, as well as native AMPK.